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Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization. Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity.


NEBuilder® Assembly Tool


Enzyme Finder

NEBuilder® Assembly Tool can be used to design primers for your Gibson Assembly reaction, based on the entered fragment sequences and the polymerase being used for amplification. Use this tool to select restriction enzymes by name, sequence, overhang or type. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job.


Double Digest Finder


DNA Sequences and Maps Tool

Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product. Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors.


Competitor Cross-Reference Tool


PCR Selection Tool

Use this tool to select another company’s competent cell product and find out which NEB strain is compatible. Choose either the product name or catalog number from the available selection, and this tool will identify the recommended NEB product and its advantages. A link to the product page where you can also order the product is provided. Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction.


Tm Calculator


NEBcutter® V2.0

Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions. Use this tool to identify the restriction sites within your DNA sequence. Choose between Type II and commercially available Type III restriction enzymes to digest your DNA. NEBcutter® V2.0 will indicate cut frequency and methylation state sensitivity.


NEB Golden Gate Assembly Tool



Use this tool to assist with in silico DNA construct design for Golden Gate DNA assembly. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings. UseNEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5® Site-Directed Mutagenesis Kit. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches.





Use this tool as a guide to the ever-changing landscape of restriction enzymes. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins. Polbase is a repository of biochemical, genetic, and structural information about DNA Polymerases.



Inteins are in-frame sequences that disrupt the coding region of a host gene. Unlike introns, inteins are excised post-translationally from a protein precursor by a self-catalytic protein splicing mechanism. InBase describes how inteins work and is home to the “Intein Registry” that includes a list of inteins and their properties, such as prototype intein, domain of life, endonuclease activity or motifs, locations of exteins, insertion site comments, discoverers (with contact information) and references.